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Opentrons Documentation
Opentrons Flex Instruction Manual
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Opentrons Flex
Modules
Protocol Designer
Python API
Opentrons Documentation
Opentrons Flex
Opentrons Flex
Preface
Introduction
Safety and Regulatory Information
Installation and Relocation
Installation and Relocation
Installation Requirements
Unboxing
First Run
Instrument Installation and Calibration
Relocation
System Description
System Description
Robot Components
Pipettes
Gripper
Emergency Stop Pendant
Connections
System Specifications
Modules
Modules
Module Caddy System
Module Calibration
Absorbance Plate Reader
Heater-Shaker
HEPA/UV Module
Magnetic Block
Stacker Module
Temperature Module
Thermocycler
Labware
Labware
Labware Concepts
Labware Types
Using Labware with the Gripper
Labware Definitions
Protocol Development
Protocol Development
Protocol Library
Custom Protocol Service
Protocol Designer
Opentrons AI
Python Protocol API
Converting OT-2 Protocols
Touchscreen
Touchscreen
Dashboard
Deck Configuration
Protocol Management
Protocol Details
Protocol Setup
Protocol Run
Quick Transfer
Instrument Management
Robot Settings
Opentrons App
Advanced Operations
Advanced Operations
Flex Log Files
Command Line Operation Over SSH
Jupyter Notebook
Maintenance
Maintenance
Cleaning Flex
Service
Appendices
Appendices
Glossary
Additional Documentation
Open-Source Software
Support and Contact Information
Modules
Modules
Flex Stacker
Flex Stacker
Safety and Compliance
Product Specifications
Pre-installation Information
Installing the Stacker
Post-installation Procedures
Loading Labware
Maintenance and Cleaning
Additional Product Information
Absorbance Plate Reader Module
Absorbance Plate Reader Module
Safety Information
Product Specifications
Deck Placement
Installing the Plate Reader
Using Plate Reader Data
Maintenance and Cleaning
Additional Product Information
HEPA/UV Module
HEPA/UV Module
Safety Information and Regulatory Compliance
Product Specifications
HEPA Specifications
UV Specifications
Unboxing and Installation
Hardware Controls
Maintenance
Cleaning
Additional Product Information
Thermocycler Module
Thermocycler Module
Safety and Regulatory Compliance
Product Specifications
Installing the Thermocycler
Installing the Thermocycler
Before You Begin
Flex Attachment Steps
OT-2 Attachment Steps
Thermocycler Lid Seals
Maintenance and Cleaning
Additional Product Information
Heater-Shaker Module
Heater-Shaker Module
Safety Information and Regulatory Compliance
Product Specifications
Thermal Adapters
Installing the Heater-Shaker
Installing the Heater-Shaker
Before You Begin
Flex Attachment Steps
OT-2 Attachment Steps
Software Control
Maintenance and Cleaning
Additional Product Information
Temperature Module
Temperature Module
Safety Information and Regulatory Compliance
Product Specifications
Installing the Temperature Module
Installing the Temperature Module
Before You Begin
Flex Installation Steps
OT-2 Installation Steps
Maintenance and Cleaning
Additional Product Information
Protocol Designer
Protocol Designer
About Protocol Designer
Create a protocol
Create a protocol
Robot and instruments
Modules and fixtures
Protocol metadata and overview
Protocol starting deck
Protocol steps
Protocol steps
Transfer steps
Mix steps
Move steps
Module steps
Magnet steps
Pause steps
Editing steps
Warnings and errors
Export a protocol
Modify an existing protocol
Python API
Python API
Tutorial
Versioning
Labware
Moving Labware
Hardware Modules
Hardware Modules
Module Setup
Absorbance Plate Reader Module
Flex Stacker Module
Heater-Shaker Module
Magnetic Block
Magnetic Module
Temperature Module
Thermocycler Module
Multiple Modules of the Same Type
Deck Slots
Pipettes
Pipettes
Loading Pipettes
Pipette Characteristics
Partial Tip Pickup
Volume Modes
Liquid Classes
Liquid Class Definitions
Building Block Commands
Building Block Commands
Manipulating Pipette Tips
Liquid Control
Utility Commands
Complex Commands
Complex Commands
Sources and Destinations
Order of Operations
Complex Command Parameters
Labware and Deck Positions
Runtime Parameters
Runtime Parameters
Choosing Parameters
Defining Parameters
Using Parameter Values
Use Case: Sample Count
Use Case: Dry Run
Use Case: Cherrypicking
Parameter Style Guide
Advanced Control
Advanced Control
Jupyter Notebook
Command Line
Robot Motor Control
Example Protocols
Adapting from OT-2 to Flex
API Reference
API Reference
Protocols
Instruments
Robot Motors
Labware and Trash Containers
Wells and Liquids
Modules
Modules
Absorbance Plate Reader
Flex Stacker
Heater-Shaker
Magnetic Block
Magnetic Module
Temperature Module
Thermocycler
Useful Types
Execute and Simulate
Instruction Manual
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Opentrons Labworks Inc.
October 2025
