Instrument Installation and Calibration

After the initial robot setup, your next step is to attach and calibrate a pipette. The OT-2 has two pipette mounts, each can hold a single-channel or multi-channel pipette. The information in this section will help you get started; the Opentrons App provides detailed, illustrated instructions about the attachment and calibration workflows. To begin:

  1. Prepare your robot for installation by removing any labware from the deck and clearing the working area to make attachment and calibration easier.

  2. In the Opentrons App, click the Devices tab and select the OT-2 you want to work with.

  3. In the Pipettes and Modules section for your device:

    • Select the left or right pipette mount.
    • Click the three-dot menu (⋮), and then click Attach pipette. The gantry will move towards the front of the deck and the mount will lower.

    pipette attachment

  4. In the Opentrons App, follow the on-screen instructions to attach and calibrate your pipette.