Mix steps

In a mix step, the robot mixes liquid by repeatedly aspirating and dispensing. Mixing occurs in each well you select, one after the other, without moving any liquid between wells.

You can customize settings for your mix step in a two-part form. Just like in a transfer step, start by selecting a pipette, tip rack, and labware. You can customize volume per well and the number of times the robot will mix. Choose tip management settings, or how often the robot will select a new tip, and a tip drop location before clicking Continue.

Select wells, volume, repetitions, and tip management settings in the first mix step form.

Note

If you select Tip rack as the tip drop location, the pipette will return tips to their original position in the tip rack. You won't be able to pick up these tips again in the same protocol.

In the aspirate and dispense tabs, you can adjust the flow rate, well order, and tip position within the well. Available advanced settings in a mix step include a delay after aspirating or dispensing, and blowout and touch tip after dispensing. See the advanced settings for Transfer steps for descriptions of each.

Like a transfer step, mix steps are blocked by lids on labware. Use a move step to remove lids, then add a mix step to your protocol.